Mouse Lung Tissue Slice Culture | Springer Nature Experiments
WEBPrecision-cut lung slices (PCLS) represent an ex vivo model widely used in visualizing interactions between lung structure and function. The major advantage of this technique is that the presence, differentiation state, and localization of the more.
Live imaging of alveologenesis in precision-cut lung slices reveals ...
WEBMar 12, 2019 · Precision-cut lung slices (PCLS) were obtained from mouse lungs according to the protocol described in ref. 19, with some modifications. Post-natal day 3 (P3), P7, P14 and adult C57BL/6...
Mouse Lung Tissue Slice Culture - PubMed
WEBPrecision-cut lung slices (PCLS) represent an ex vivo model widely used in visualizing interactions between lung structure and function.
Precision-cut Mouse Lung Slices to Visualize Live Pulmonary …
WEBApr 5, 2017 · In contrast to these aforementioned techniques, the location of individual cell types within the lung can be readily visualized by generating Precision-cut Lung Slices (PCLS), staining them with commercially available, fluorescently tagged antibodies, and visualizing the sections by confocal microscopy.
Precision-cut Mouse Lung Slices to Visualize Live Pulmonary …
WEBApr 5, 2017 · We have used this protocol to successfully visualize the location of a wide variety of cell types in the lung, including distinct types of dendritic cells, macrophages, neutrophils, T cells and B cells, as well as structural cells such as lymphatic, endothelial, and epithelial cells.
Precision-cut Mouse Lung Slices to Visualize Live Pulmonary
WEBApr 5, 2017 · We describe a method for generating Precision-cut Lung Slices (PCLS) and immunostaining them to visualize the localization of various immune cell types in the lung. Our protocol can be extended to visualize the location and function of many different cell types under a variety of conditions.
Acute brain slice methods for adult and aging animals: application …
WEB1. Introduction. For several decades the vast majority of brain slice physiologists have relied upon a ‘protective cutting’ method for preparing healthy brain slices from juvenile and adolescent animals.
Microdissection of Mouse Brain into Functionally and ... - JoVE
WEBFeb 15, 2021 · Introduction. The brain, along with the spinal cord and retina, comprise the central nervous system that executes complex behaviors, controlled by specialized, precisely positioned, and interacting cell types throughout the entire body 1.
A three-dimensional, population-based average of the C57BL/6 mouse …
WEBJul 13, 2020 · The data set constitutes a rich three-dimensional representation of the average mouse brain in the DAPI staining modality reconstructed from coronal slices and includes an automatic segmentation...
A simple, step-by-step dissection protocol for the rapid …
WEBFeb 11, 2016 · After dissecting out the spinal column, from the base of the skull to the level of the femurs, it can be cut down the mid-line and the spinal cord and meninges removed, before extracting the DRG and detaching unwanted axons. This protocol allows the easy and rapid isolation of DRG with minimal practice and dissection experience.
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